Dr. LOUIS SHERMAN Professor 765-494-8106 LILY G-235 lsherman@purdue.edu Associated website(s): Lab , Publications

---

PROFESSIONAL FACULTY RESEARCH

(microbiology) Photosynthetic membranes - structure, function, and assembly in cyanobacteria; functional genomics and impact of environmental factors on photosynthesis; analysis of global regulation with microarrays; regulation of photosynthesis and N ₂ -fixation in unicellular cyanobacteria.

BIO

Cyanobacteria have become wonderful and versatile model organisms for the study of photosynthesis, nitrogen fixation and responses to environmental stresses. Our research can help answer questions involved with environmental concerns, alternative energy uses (i.e., solar energy), and health concerns such as microbial toxins and the design of new drugs. The genomic sequence of one favorite cyanobacterium was completed a few years ago, and we now have the task of trying to determine the function of each of its 3,264 genes, ultimately to understand how a cell functions. The lab has successfully constructed microarrays ("gene chips")--this is an important technique in biology and all of the genes in an organism can be spotted on a glass slide about the size of a microscope slide. These microarrays are great for studying the activity of genes; this technique, along with the ability to make mutations in virtually every gene, is a key way for determining gene function under different environmental conditions. The genome sequence of another of our favorite cyanobacteria has recently been completed. This unicellular organism shows robust metabolic and circadian rhythms and performs photosynthesis and N ₂ -fixation at different times of the day and night. This organism is key to a large project aimed at understanding the regulation of such processes and the assembly of membrane complexes. The genomes of 6 more Cyanothece species are being sequenced at DOE and will aid us in identifying important genes and processes for alternate energy uses.

Education

B.S., University of Chicago, 1965

Ph.D., University of Chicago, 1970

Postdoc, Cornell University, 1972

Awards

• Lady Davis Fellowship, Hebrew University, Jerusalem, Israel
• Fellow, American Academy of Microbiology
• Fellow, American Association for Advancement of Science (AAAS)

Other Activities

Grant Review/Study Section

• DOE
• NSF
• USDA
• NIH

Presentations

Uma K. Aryal ¹ , Stephen J. Callister ¹ , Janani I. Shutthanandan ¹ , Xiaohui Zhang ² , Horgato Min ² , Sujata Mishra ² , , Dave W. Koppenaal ¹ , Richard D. Smith ¹ , Himadri B Pakrasi ³ and Louis A. Sherman. ^. 2012. Quantitative proteomic analyses reveal H ₂ production in Cyanothece strains ATCC51142 and PCC7822 under aerobic growth. DOE GtL meeting, Washington, DC, Feb. 29-Mar. 2, 2012.

Sherman, L.A. 2012.  Following the clock in the diazotrophic cyanobacterium Cyanothece. Lorentz Center Meeting on Hacking the Clock, Leiden, The Netherlands, April 9-13, 2012.

Nagarajan, S. and Sherman, L.A. 2012.  Role of the Plasmid hik31 operon in regulating carbohydrate  metabolism in the dark in Synechocystis sp. Strain PCC 6803.  ASM General Meeting, San Francisco, CA, June 17-20, 2012.

Louis A. Sherman, Anindita Bandyopadhyay, Thanura Elvitigala _, Eric Welsh, Jana Stöckel, Michelle Liberton ^, Hongtao Min, Himadri B. Pakrasi. 2012. New metabolic attributes of Cyanothece, a group of unicellular nitrogen fixing cyanobacteria.  Finishing for the Future, Santa Fe, NM, June 5-8, 2012.

Nagarajan, S. and Sherman, L.A. 2012.  Role of the two hik31 operons in regulating carbohydrate and photosynthesis metabolism in Synechocystis sp. PCC 6803.  14 ^th International Symposium on Phototrophic Prokaryotes, Porto, Portugal, Aug. 5-10, 2012.

Sarah Griffith and Louis A. Sherman, 2013. The effects of light-dark cycles on the metabolism of Cyanothece sp. ATCC 51142 and Cyanothece sp. ATCC 7822. 11 ^th Cyanobacterial Workshop, St. Louis, MO, August 7-11, 2013.

David Welkie ¹ , Rajib Saha ² , Xiaohui Zhang ¹ , Costas D Maranas ² and Louis A. Sherman ¹ _, 2013. Optimizing isoprene production in Synechocystis 6803 utilizing computational modeling and transcriptional regulation. 11 ^th Cyanobacterial Workshop, St. Louis, MO, August 7-11, 2013.

David Welkie and Louis Sherman, 2013.  The impact of media adjustments on growth and analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822. 11 ^th Cyanobacterial Workshop, St. Louis, MO, August 7-11, 2013.

David Welkie ¹ , Xiaohui Zhang ¹ , Galya Orr ² , Jon Jacobs ² and Louis A. Sherman ¹ , 2013.  Gene Expression in the unicellular, diazotrophic cyanobacterium Cyanothece sp. PCC 7822 under N ₂ -fixing conditions. 11 ^th Cyanobacterial Workshop, St. Louis, MO, August 7-11, 2013.

Xiaohui Zhang and Louis Sherman, 2013.  Characterization of an uptake hydrogenase deletion mutant strain of Cyanothece sp. PCC 7822. 11 ^th Cyanobacterial Workshop, St. Louis, MO, August 7-11, 2013.

David Welkie and Louis Sherman. 2014.  Modifying Energy Storage in cyanobacteria: from glycogen to starch.  9 ^th European Workshop on Molecular Biology of Cyanobacteria. Texel, The Netherlands, Sept. 7-11, 2014.

Sherman, Louis A. 2016.  Systems Biology of a diazotrophic cyanobacterium of the genus Cyanothece .  Systems Biology of Cyanobacteria Workshop, July 18-20, 2016, Berlin, Germany.

Sherman, Louis A. 2016.  Altering the structure of carbohydrate storage granules in the cyanobacterium Synechocystis sp. PCC 6803. U. of Lille, Lille, France.  July 15-16, 2016.

Sherman, Louis A. 2016.  Systems Biology of a diazotrophic cyanobacterium of the genus Cyanothece . Beijing Univ. of Chem Tech. Symposium., Beijing, China.  May 29, 2016.

Sherman, Louis A., David G. Welkie and Byung-Hoo Lee.  2016. Altering the structure of carbohydrate storage granules in the cyanobacterium Synechocystis sp. PCC 6803. 12 ^th Cyanobacterial Workshop, Tempe, AZ, May 19-22, 2016.

Published Papers

Min, H. and Sherman, L.A.  2010.  Genetic transformation and mutagenesis via ssDNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece .  Appl. Environ. Microbiol., 76 :7641-7645.

Bandyopadhyay, A., Stöckel, J., Min, H., Sherman, L.A. and Pakrasi, H.B. 2010. High Rates of   phtotobiological H ₂ production by a cyanobacteriium under aerobic conditions.  Nature Comm. 1: 139, 1-7.

Summerfield, T.C., Nagarajan, S. and Sherman, L.A.  2011.  Gene expression under low oxygen conditions and the role of the histidine kinase, Hik31 (Sll0790), in the cyanobacterium Synechocystis sp. PCC 6803.  Microbiology, 157:1-12.

McDermott, J.E. Oehmen, C., McCue, L.A., Hill, E., Choi, D., Stockel, J., Liberton, M., Pakrasi,

H.B., and Sherman, L.A. 2011.  A model of cyclic transciptomic behavior in the cyanobacterium Cyanothece sp. ATCC 51142.  Molecular BioSystems, 7:2407-2418.

Bandyopadhyay, A., Elvitigala, T. Welsh,E., Stöckel, J., Liberton,  M., Min, H. Sherman, L.A.,  and    Pakrasi, H.B.  2011. Novel   metabolic attributes of Cyanothece , a group of unicellular nitrogen fixing cyanobacteria. mBio 2:e00214-11.

Nagarajan, S., Sherman, D.M., Shaw, I. and Sherman, L.A. 2012.  Functions of the duplicated hik31 operons in central metabolism and responses to light, dark, and carbon sources in Synechocystis sp. Strain PCC 6803.  J. Bacteriol. 194:448-459.

Wang, Q., Singh, A, Li, H., Nedbal, L., Sherman, L.A., Govindjee and Whitmarsh, J. 2012.  Net light-induced oxygen evolution in photosystem I deletion mutants of the cyanobacterium Synechocystis sp. PCC 6803.  Biochimica et Biophysica Acta Bioenergetics, 1817:792-801.

Zhang, Xiaohui and Sherman, L.A. 2012. Alternate copies of D1 are used by cyanobacteria under different environmental conditions.  Photosynth Res. 114:133-135.

Uma K. Aryal, Stephen J. Callister, Sujata Mishra, Xiaohui Zhang, Janani I. Shutthanandan, Thomas E. Angel, Anil K. Shukla, Matthew E. Monroe, Ronald J. Moore, David W. Koppenaal, Richard D.   Smith   and Louis Sherman, 2013. Proteome Analyses of Strains Cyanothece ATCC 51142 and PCC 7822 of the Diazotrophic Cyanobacterium Cyanothece sp. under Culture Conditions  Resulting in Enhanced H ₂ Production. Appl. Environ. Microbiol . 2013, 79(4):1070-1077.

Summerfield, T., Crawford TS, Young RD, Chua JPS, Macdonald, RL, Sherman LA and Eaton-Rye, JJ. 2013. Environmental pH Affects Photoautotrophic growth of Synechocystis sp. PCC 6803 strains carrying mutations in the lumenal proteins of PSII. Plant Cell Physiol. 2013 54:859-874.

Cerveny, J, Sinetova, MA, Valledor, L, Sherman, LA and Nedbal, L. 2013.  Ultradian metabolic   rhythm in the diazotrophic cyanobacterium Cyanothece sp. ATCC 51142. Proc. Natl. Acad Sci. 110: 13210-13215.

Welkie, DG, Sherman, DM, Chrisler, WB, Orr, G and Sherman, LA. 2013.  Analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822.  Photosyn Res. 118:25-36.

Nagarajan, S., Srivastava, S., and Sherman, L.A. 2014.  Essential role of he plasmid hik31 operon in regulating central metabolism in the dark in S ynechocystis sp. PCC 6803.  Molec. Microbiol, 91:79-97.

Zhang, X., Sherman, D. M. and Sherman, L.A. 2014. The uptake hydrogenase in the unicellular diazotrophic cyanobacterium Cyanothece sp. Strain PCC 7822 protects nitrogenase from oxygen toxicity. J. Bacteriol. 196: 840-849.

Aryal, UK, Callister, SJ, McMahon, BH, McCue, L-A, Brown, J, Stöckel, J, Liberton, M, Mishra, S, Zhang, X, Nicora, CD, Angel, TE, Koppenaal, DW, Smith, DW, Pakrasi, HB, and Sherman, LA. 2014.  Proteomic screens of five strains of oxygenic photosynthetic cyanobacteria of the genus Cyanothece. J Proteome Research 13:3262-3276.

Arshad, S., Mishra, S. and Sherman L.A. 2014.  The effects of different light-dark cycles on the diazotrophic, unicellular cyanobacteria Cyanothece sp. ATCC 51142 and Cyanothece sp. PCC 7822. J. Phycology 50:930-938.

David Welkie, Xiaohui Zhang, Meng Lye Markillie, Ronald Taylor, Galya Orr, Jon Jacobs, Ketaki Bhide, Jyothi Thimmapuram, Hugh Mitchell, and Louis A. Sherman.  2014.  Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 across a 24 hour light-dark cycle.  BMC Genomics15:1185, 1-16.

Welkie, D.G., Lee, B-H and Sherman, L.A. 2016.  Altering the Structure of carbohydrate Storage Granules in the Cyanobacterium Synechocystis sp. Strain PCC 6803 through Branching-Enzyme Truncations. J. Bacteriol. 198:701-710.

Summerfield, TC, Toepel, J and Sherman, LA. 2011.  Transcriptional Response to low O ₂ conditions in Synechocystis sp. Strain PCC 6803 includes induction of a gene cluster containing psbA and petC2 . Photosynthesis in the Global Perspective (Kadur, GN, editor), pp. 120-129.

Sherman, L.A., Min, H, Toepel, J and Pakrasi, H.B. 2010.  Better living through Cyanothece unicellular diazotrophic cyanobacteria with highly versatile metabolic systems. Recent Advances in Phototrophic Prokaryotes (Hallenbeck, P.C., editor), pp. 275-290, Springer, New York.

Michael Montague*, George H. McArthur IV, Charles S. Cockell, Jason Held, William Marshall,   Louis A Sherman, Norman Wang, Wayne L. Nicholson, Daniel R., Tarjan, John Cumbers. 2012. The role of synthetic biology for in situ resource utilization (ISRU).  Astrobiology, 12: doi: 10-1089/ast.2012.0829.

Louis A. Sherman, Pramod P. Wangikar, Renu Swarup and Sangita Kasture. 2013.  Highlights from the Indo-US workshop “Cyanobacterial molecular networks to biofuels” held at Lonavala, India during December 16-20, 2012. Photosynth. Res. 118: 1-8.

Aryl, Uma K. and Sherman, Louis A. 2017.  Transcriptomic and Proteomic Analysis to understand systems-level properties of diurnal cycles in nitrogen-fixing cyanobacteria. Cyanobacteria: Omics and Manipulation (Los, Dmitry A, editor), pp. 117-144, Caister Academic Press, UK.

Conferences

• 6 ^th NCSE Conf: Energy for a Sustainable and Secure Future, January 2006
• AAAS Meeting and Symposium on Grand Challenges, St. Louis, MO, February 2006
• 1 ^st Annual Finishing Genomes in the Future Meeting, Santa Fe, NM, May 2006
• 106 ^th ASM Meeting, Orlando, FL, May 2006
• 12 ^th International Symposium on Phototrophic Prokaryotes, Pau, France, August 2006
• 15 ^th International Nitrogen Fixation Congress, Cape Town, South Africa, January 2007
• Membrane Biology Grand Challenge Workshop, St. Louis, MO, April 2007
• 9 ^th Cyanobacterial Workshop on Molecular Biology, Delavan, WI, June 2007
• 2 ^nd Annual Finishing Genomes in the Future Meeting, Santa Fe, NM, June 2007
• Avron-Minerva Symposium on Photosynthesis, Jerusalem, Israel, November 2007
• Society for General Microbiology, Edinburgh, Scotland, April 2008
• Photosynthesis in the Global Perspective, Indore, India, November 2008
• DOE Genomics Sciences Meeting, Washington, DC, February 2009
• University of Stockholm, Department Plant Biology, May 2009
• 13th International Symposium on Phototrophic Prokaryotes, Montreal, Canada, August 2009
• Algal CO ₂ Capture, St. Louis, MO, September 2009
• DOE Genomics Sciences Meeting, Washington, DC, February 2010
• DOE Joint Genome Initiative Genomics Meeting, Walnut Creek, California, March 2010
• Sequencing, Finishing and Analysis in the Future, Santa Fe, New Mexico, June 2010
• 10th Molecular Biology of Cyanobacteria Workshop, Lake Arrowhead, California, June 2010
• NASA - Synthetic Biology, Ames, California, October 2010
• Modeling Cyanobacteria in silico : Virtual Bioreactor, Mikulov, Czech Republic, September 2010
• DOE Genomics Sciences/Biofuels Meeting, Washington, DC, April 2011
• ASM Meeting, New Orleans, Louisiana, May 2011
• Workshop on Cyanobactrial Metabolism, Lake Geneva, Wisconsin, June 2011
• 8 ^th European Workshop on Mol Biol Cyanobacteria, Naantali, Finland, August 2011
• ESF Conf. Microorganisms for BioFuel Production, Bielefeld, Germany, September 2011