Bimolecular fluorescence complementation (BMFC) to investigate protein-protein interactions in planta

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For additional information about this research, contact:

Dr. Stanton Gelvin gelvin@bilbo.bio.purdue.edu
765/494-4939
Department of Biological Sciences Purdue University 1392 Lilly Hall West Lafayette, IN 47907-1392

Protocols and Vectors

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Bimolecular Fluorescence Complementation for Imaging Protein Interactions in Plant Hosts of Microbial Pathogens
By Lan-Ying Lee and Stanton B. Gelvin

Vectors for marker gene fusions

• pSAT4A-mCherry-N1 (pE3279) map
• pSAT4A-mCherry-N1 (pE3279) seq
• pSAT6-EYFP-N1 (pE3225) map
• pSAT6-EYFP-N1 (pE3225) seq
• pSAT6-EYPF-C1 (pE3150) map
• pSAT6-EYPF-C1 (pE3150) seq
• pSAT6-mCherry-C1-B (pE3275) map
• pSAT6-mCherry-C1-B (pE3275) seq
• pSAT6-RFP-C1-map
• pSAT6-RFP-C1-seq
• pSAT6-RFP-N1-map
• pSAT6-RFP-N1-seq

BiFC vectors

• pSAT1-cEYFP-C1-B-map
• pSAT1-cEYFP-C1-B-seq
• pSAT1-cEYFP-N1-map
• pSAT1-cEYFP-N1-seq
• pSAT1-nEYFP-C1-map
• pSAT1-nEYFP-C1-seq
• pSAT1-nEYFP-N1-map
• pSAT1-nEYFP-N1-seq
• pSAT1A-cEYFP-N1-map
• pSAT1A-cEYFP-N1-seq
• pSAT1A-nEYFP-N1-map
• pSAT1A-nEYFP-N1-seq
• pSAT4-cEYFP-C1-B-map
• pSAT4-cEYFP-C1-B-seq
• pSAT4-cEYFP-N1-map
• pSAT4-cEYFP-N1-seq
• pSAT4-nEYFP-C1-map
• pSAT4-nEYFP-C1-seq
• pSAT4-nEYFP-N1-map
• pSAT4-nEYFP-N1-seq
• pSAT4A-cEYFP-N1-map
• pSAT4A-cEYFP-N1-seq
• pSAT4A-nEYFP-N1-map
• pSAT4A-nEYFP-N1-seq
• pSAT6-cEYFP-C1-B (E3108) map
• pSAT6-cEYFP-C1-B (E3108) seq
• pSAT6-cEYFP-N1 ( E2914) map
• pSAT6-cEYFP-N1 (E2914) seq
• pSAT6-nEYFP-C1 (E2884) map
• pSAT6-nEYFP-C1 (E2884) seq
• pSAT6-nEYFP-N1 (E2913) map
• pSAT6-nEYFP-N1 (E2913) seq

Gateway BiFC Vectors

• pSAT4-DEST-n(174)EYFP-Cl (pE3136) seq
• pSAT4-DEST-nEYFP-C1 (pE3136) map
• pSAT4(A)-DEST-n(1-174)EYFP-N1 (pE3134) seq
• pSAT4(A)-DEST-nEYFP-N1 (pE3134) map
• pSAT5-DEST-c(175-end)EYFP-C1(B) (pE3130) seq
• pSAT5-DEST-cEYFP-C1(B) (pE3130) map
• pSAT5(A)-DEST-c(175-end)EYFP-N1 (pE3132) seq
• pSAT5(A)-DEST-cEYFP-N1 (pE3132) map

Multicolor BiFC Vectors

• pSAT1-cCFP-C (pE3242) map
• pSAT1-cCFP-C (pE3242) seq
• pSAT1-cCFP-N (pE3449) map
• pSAT1-cCFP-N (pE3449) seq
• pSAT1-nCerulean-C (pE3415) map
• pSAT1-nCerulean-C (pE3415) seq
• pSAT1-nCerulean-N (pE3307) map
• pSAT1-nCerulean-N (pE3307) seq
• pSAT1-nVenus-C (pE3228) map
• pSAT1-nVenus-C (pE3228) seq
• pSAT1-nVenus-N (pE3308) map
• pSAT1-nVenus-N (pE3308) seq
• pSAT1A-cCFP-N (pE3450) map
• pSAT1A-cCFP-N (pE3450) seq
• pSAT1A-nCerulean-N (pE3246) map
• pSAT1A-nCerulean-N (pE3246) seq
• pSAT1A-nVenus-N (pE3231) map
• pSAT1A-nVenus-N (pE3231) seq
• pSAT4-cCFP-C (pE3243) map
• pSAT4-cCFP-C (pE3243) seq
• pSAT4-cCFP-N (pE3451) map
• pSAT4-cCFP-N (pE3451) seq
• pSAT4-nCerulean-C (pE3416) map
• pSAT4-nCerulean-C (pE3416) seq
• pSAT4-nCerulean-N (pE3309) map
• pSAT4-nCerulean-N (pE3309) seq
• pSAT4-nVenus-C (pE3229) map
• pSAT4-nVenus-C (pE3229) seq
• pSAT4-nVenus-N (pE3310) map
• pSAT4-nVenus-N (pE3310) seq
• pSAT4A-cCFP-N (pE3347) map
• pSAT4A-cCFP-N (pE3347) seq
• pSAT4A-nCerulean-N (pE3247) map
• pSAT4A-nCerulean-N (pE3247) seq
• pSAT4A-nVenus-N (pE3232) map
• pSAT4A-nVenus-N (pE3232) seq
• pSAT6-cCFP-C (pE3244) map
• pSAT6-cCFP-C (pE3244) seq
• pSAT6-cCFP-N (pE3497) map
• pSAT6-cCFP-N (pE3497) seq
• pSAT6-nCerulean-C (pE3417) map
• pSAT6-nCerulean-C (pE3417) seq
• pSAT6-nCerulean-N (pE3248) map
• pSAT6-nCerulean-N (pE3248) seq
• pSAT6-nVenus-C (pE3230) map
• pSAT6-nVenus-C (pE3230) seq
• pSAT6-nVenus-N (pE3233) map
• pSAT6-nVenus-N (pE3233) seq

T-DNA Binary Vectors

• pE3055 map
• pE3055 seq
• pE3184 map
• pE3184 seq
• pE3185 map
• pE3185 seq
• pPZP-RCS2-ocs-bar-RI (pE3519) map
• pPZP-RCS2-ocs-bar-RI (pE3519) seq
• pPZP-RCS2-ocs-bar-Sma (pE3407) map
• pPZP-RCS2-ocs-bar-Sma (pE3407) seq

This work is made possible by a grant from the National Science Foundation's Arabidopsis 2010 Research Program