Bimolecular fluorescence complementation (BMFC) to investigate protein-protein interactions in planta

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For additional information about this research, contact:

Dr. Stanton Gelvin gelvin@bilbo.bio.purdue.edu
765/494-4939
Department of Biological Sciences Purdue University 1392 Lilly Hall West Lafayette, IN 47907-1392

Data

BiFC
Slide Show

• nYFP-VirD2 and various isoforms of importin a-cYFP interact and localize to the nucleus of tobacco BY-2 cells
• VirE2-nYFP and VirE2-cYFP interact and localize to the cytoplasm of tobacco BY-2 cells
• VirE2-nYFP and various isoforms of importin a-cYFP interact and localize to the cytoplasm of tobacco BY-2
• Importin a proteins dimerize and localize to the nucleus of tobacco BY-2 cells and onion cells

 

 

 

 

 

 

This work is made possible by a grant from the National Science Foundation's Arabidopsis 2010 Research Program