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Richard J. Kuhn Lab

Publications

PubMed Listing

2021

  • Ghosh, A. K., D. Shahabi, M. Yadav, S. Kovela, B. J. Anson, E. K. Lendy, C. Bonham, D. Sirohi,A. Brito-Sierra, S. Hattori, R. Kuhn, H. Mitsuya and A. D. Mesecar. 2021. Chloropyridinyl esters of nonsteroidal anti-inflammatory agents and related derivatives as potent SARS-CoV-2 3CL protease inhibitors. Molecules, accepted.

  • Cao, T.M. and R. J. Kuhn.  2021.  Chapter 7.  Construction of genomic and sub-genomic dengue infectious replicons.  In:  Methods in Molecular Biology.  Dengue Virus: Methods andProtocols, R. Mohana-Borges (ed), Humana Press, in press. 10.1007/978-1-0716-1879-0

  • Munnamalai, V., N. H. Sammudin, C. A. Young, A. Thawani, R. J. Kuhn and D. M. Fekete. 2021. Embryonic and neonatal mouse cochleae are susceptible to Zika virus infection. Viruses. Sep 14;13(9):1823. Doi: 10:3390/v13091823. PMID: 34578404.

  • Wessel, A., K. Dowd, S. Biering, P. Zhang, M. Edeling, C. Nelson, K. Funk, C. DeMaso, R. Klein,Smith, T. Cao, R. Kuhn, D. Fremont, E. Harris, T. Pierson and M. Diamond. 2021. Levels of circulating NS1 impact West Nile virus spread to the brain. J Virol. 2021 Aug 4:JVI0084421. doi: 10.1128/JVI.00844-21. Epub ahead of print. PMID: 34346770.

  • Khare, B., T. Klose, Q. Fang, M. G. Rossmann and R. J. Kuhn.  2021.  Structure of Usutu virus SAAR-1776 displays fusion loop asymmetry.  PNAS, Aug. 24, 2021, 118 (34) e2107408118. https://doi.org/10.1073/pnas.2107408118. PMID: 34417300; PMCID: PMC8403871.

  • Sevvana, M., Z. Keck, S. K. H. Foung, and R. J. Kuhn. 2021.  Structural perspectives on HCV humoral immune evasion mechanisms. Curr. Opin. Virol. Aug;49:92-101. doi.org/10.1016/j.coviro.2021.05.002. Epub 2021, Jun 3. PMID:34091143; PMCID:PMC8319080.

2020

  • Nicholls, C. M. R., M. Sevvana and R. J. Kuhn.  2020. Structure-guided paradigm shifts in flavivirus assembly and maturation mechanism. 108:33-83. In: Advances in Virus Research, M.Kielian, T. C. Mettenleiter, M. J. Roossinck (eds), Academic Press

  • Sevvana, M., T. F. Rogers, A. S. Miller, F. Long, T. Klose, N. Beutler, Y-C. Lai, M. Parren, L. M. Walker, G. Buda, D. R. Burton, M. G. Rossmann and R. J. Kuhn.  2020. Structural basis of Zika Virus specific neutralization in subsequent Flavivirus infections. Viruses 12(12):1346. Doi:10.3390/v12121346. PMID: 33255202; PMCID: PMC7760643.

  • Coelho, D. R., P. H. Carneiro, L. Mendes-Monteiro, J. N. Conde, I. Andrade, T. Cao, D. Allonso,White-Dibiasio, R. J. Kuhn and R. Mohana-Borges. 2021. ApoA1 neutralizes pro inflammatory effects of Dengue virus NS1 protein and modulates the viral immune evasion.  J. Viroll, June10;95(13):e0197420. Doi: 10.1128JVI.01974-20. PMID: 33827950.

  • Thawani, A., N. H. Sammudin, H. S. Reygaerts, A. N. Wozniak, V. Munnamalai, R. J. Kuhn and M. Fekete. 2020. Zika virus can directly infect and damage the auditory and vestibular components of the embryonic chicken inner ear.  Dev Dyn. Jul;249(7):867-883. Doi:10.1002/dvdy.176. Epub 2020 May 8. PMID: 32384225; PMCID: PMC7753925. Doi.org/10.1916/bs.aivir.2020.08.003

  • Powell, L. A., A. Miller, J. M. Fox, N. Kose, T. Klose, A. S. Kim, R. Bombardi, R. N. Tennekoon, A. Dharshan de Silva, R. H. Carnahan, T. E. Morrison, M. S. Diamond, M. G. Rossmann, R. J. Kuhn and J. E. Crowe. 2020. Human mAbs broadly protect against arthritogenic alphaviruses by recognizing conserved elements of the Mxra8 receptor-binding site. Cell Host Microbe. 28(5):699-711.e7. doi: 10:1016/J. chom.2020.07.008. Epub 2020 Aug. 11. PMID: 32783883; PMCID: PMC7666055.

  • Srivastava, M, Y Zhang, J Chen, et al. 2020. Chemical proteomics tracks virus entry and uncovers NCA as Zika virus receptor. Nat Commun. 11(1):3896. Doi:10.1028.s41467-020-17638-y. PMID: 32753727;PMCID: PMC7403387.  

  • Vogt, M. R., J. Fu, N. Kose, L. E. Williamson, R. Bombardi, I. Setliff, I. S. Georgiev, T. Klose, M. G. Rossmann, Y. A. Bockkov, J. E. Gern, R. J. Kuhn and J. E. Crowe, Jr.  2020.  Human antibodies neutralize Enterovirus D68 and protect against infection and paralytic disease. Sci. Immunol. Jul 3;5(49):eaba4902. doi: 10.1126/sciimmunol.aba4902.PMID: 32620559; MCID: PMC7418079.

  • Sevvana, M and Kuhn RJ.  2020. Mapping the diverse structural landscape of the flavivirus antibody repertoire. [published online ahead of print, 2020 Aug. 12] Curr. Opin. Virol. 45:51-64. Doi:10:1016/j.coviro.2020.07.06. PMID:32801077.

  • Fatma B, Kumar R, Singh VA, et al. 2020. Alphavirus capsid protease inhibitors as potential antiviral agents for Chikungunya infection. Antiviral Res. 179:104808. PMID: 32380148 doi:10.1016/j.antiviral.2020.104808

  • Sun Y, Watters K, Hill MG, et al. 2020. Cryo-EM structure of rhinovirus C15a bound to its cadherin-related protein 3 receptor. Proc Natl Acad Sci 117(12):6784‐6791. PMID: 32152109 doi:10.1073/pnas.1921640117. PMID: 32152109; PMCID: PMC7104174.

2019

  • Kesari AS, Heintz VJ, Poudyal S, Miller AS, Kuhn RJ, LaCount DJ. Zika virus NS5 localizes at centrosomes during cell division. Virology. 2020;541:52‐62. PMID: 32056715; PMCID: PMC7216507 doi:10.1016/j.virol.2019.11.018

  • Trauchessec, M., O. Lambert, P. Bonnafous, et al.  2019. Complementary use of mass spectrometry and cryo-electron microscopy to assess the maturity of live attenuated dengue vaccine viruses. Vaccine, https://doi.org/10.1016/j.vaccine.2019.05.012.

2018

  • Therkelsen, M.D., T. Klose, F. Vago, W. Jiang, M.G. Rossmann and R.J. Kuhn. 2018.  Flaviviruses have imperfect icosahedral symmetry.  Proc. Natl. Acad. Sci.,115:11608-11612. PMID: 30348794. PMCID: PMC6233072.

  • Long, F., M. Doyle, E. Fernandez, A. S. Miller, T. Klose, M. Sevvana, A. Bryan, E. Davidson, B. J. Doranz, R. J. Kuhn, M. S. Diamond, J. E. Crowe, Jr., and M. G. Rossmann.  2019.  Structural basis of a potent human monoclonal antibody against Zika virus targeting a quaternary epitope.  PNAS 116:1591-1596. PMID: 30642974; PMCID: PMC6358714. https://doi.org/10.1073/pnas.1815432116

  • Sevvana, M., F. Long, A.S. Miller, T. Klose, G. Buda, L. Sun, R.J. Kuhn and M.G. Rossmann.  2018.  Refinement and analysis of the mature Zika virus cryo-EM structure at 3.1 Å resolution.  Structure 26:1169-1177. PMID:29958768; PMCID: PMC6125166.

  • Narwal, M., H. Singh, S. Pratap, A. Malik, R. J. Kuhn, P. Kumar, and S. Tomar.  2018.  Crystal structure of chikungunya virus nsP2 cysteine protease reveals a putative flexible loop blocking its active site. Int. J. Mol. Macromol. 116:451-462.  PMID: 29730006. DOI: 10.1016/j.ljbiomac.2018.05.007.

  • Thawani, A., D. Sirohi, R. J. Kuhn and D. M. Fekete.  2018.  Zika virus can strongly infect and disrupt secondary organizers in the ventricular zone of the embryonic chicken brain.  Cell Reports. 23(3):692-700. PMID: 29669275; PMCID: PMC6082411.

  • Mendes, A. and R. J. Kuhn.  2018.  Alphavirus nucleocapsid packaging and assembly.  Viruses 10(3):138. PMID:29558394; PMCID:PMC5869531

  • Chotiwan, N., B.G. Andre, I. Sanchez-Vargas, M.N. Islam, J.M. Grabowski, A. Hopf-Jannasch, et al.  2018.  Dynamic remodeling of lipids coincides with dengue virus replication in the midgut of Aedes aegypti mosquitoes. PLoS 14(2):e1006853. PMID:29447265; PMCID:PMC5814098

  • Hasan, S.S., M. Sevvana, R. J. Kuhn, and M. G. Rossmann. 2018.  Structural biology of Zika virus and other flaviviruses.  Nat. Struct. Mol. Biol. 25:13-20. PMID: 29323278.  DOI: 10.1038/s41594-017-0010-8.

  • Goo, L., C.R. DeMaso, R.S. Pelc, J.E. Ledgerwood, B.S. Graham, R.J. Kuhn, and T.C. Pierson.  2018.  The Zika virus envelope protein glycan loop regulates virion antigenicity.  Virology, 515:191-202.  PMID: 29304471.  DOI: 10.1016/j.virol.2017.12.032.

2017

  • Sirohi, D., R. J. Kuhn. 2017. Zika virus structure, maturation and receptors. J. Infect. Dis. 216:S10:S935-944. PMID: 29267925. DOI: 10.1093/infdis/jix515

  • Sirohi, D., R. J. Kuhn.  2017. Can an FDA-approved Alzheimer’s drug be repurposed for alleviating neuronal symptoms of Zika virus?  mBio 8e00916-17.  PMID: 28655823 PMCID: PMC5487734.

  • Hasan, S.S., A. Miller, G. Sapparapu, E. Fernandez, T. Klose, F. Long, A Fokin, J.C. Porta, W. Jiang, M.S. Diamond, J.E. Crowe, Jr., R.J. Kuhn and M.G. Rossmann.  2017.  A human antibody against Zika virus crosslinks the E protein to prevent infection.  Nat. Commun. 8:14722. PMID: 28300075; PMCID: PMC5356071

  • Jose, J., A. B. Taylor and R. J. Kuhn.  2017.  Spatial and temporal analysis of alphavirus replication and assembly in mammalian and mosquito cells”.  mBio 8:e02294-16. PMID: 28196962. PMCID PMC5312085.

  • Prasad, V.M., A. S. Miller, T. Klose, D. Sirohi, G. Buda, W. Jiang, R. J. Kuhn and M. G. Rossmann.  2017.  Structure of the immature Zika virus at 9 Å resolution. Nat. Struct. Mol. Biol. 24:184-186. PMID: 28067914. PMCID PMC5296287.

  • Grabowski, J. M., M. Guila-Nuss, R. J. Kuhn, and C. A. Hill.  2017.  RNAi reveals proteins for metabolism and protein processing associated with Langat virus infection in lxodes scapularis ISE6 cells.  Parasit. Vectors, 10:24. PMID:28086865. PMCID: PMC5237174.

2016

  • Jin, S.-A., S. Poudyal, E. E. Marinero, R. J. Kuhn and L. A. Stanciu.  2016.  Impedimetric Dengue biosensor based on functional graphene oxide wrapped silica particles.  Electrochimica Acta., 194:422-240.

  • Brown, W. C., D. L. Akey, J. R. Konwerski, J. T. Tarrasch, G. Skiniotis, R. J. Kuhn and J. L. Smith. 2016.  Extended surface for membrane association in Zika virus NS1 structure.  Nat. Struct. Mol. Biol., 23:865-867. PMID 27455458.

  • Mukherjee, S., D. Sirohi, K. A. Dowd, Z. Chen, M. S. Diamond, R. J. Kuhn, and T. C. Pierson. 2016.  Enhancing dengue virus maturation using a stable furin over-expressing cell line.  Virology 497:33-40. PMID: 27420797.  PMCID: PMC5502530.

  • Sirohi D., Z. Chen, L. Sun, T. Klose, T. C. Pierson, M. G. Rossmann and R. J. Kuhn. 2016. The 3.8Å resolution Cryo-EM structure of Zika Virus. Science 352:467-470. DOI: 10.1126/science.aaf5316.  PMID: 27033547; PMCID PMC4845755.

  • Perera, R. and R. J. Kuhn.  2016.  Chapter 4. “Host metabolism and its contribution in Flavivirus biogenesis” In: Arboviruses: Molecular Biology, Evolution and Control, D. Gubler and N. Vasilakis (eds), Caister Acad. Press.

  • Grabowski, J. M., R. Perera, A. M. Roumani, V. E. Hedrick, H. D. Inerowicz, C. A. Hill and R. J. Kuhn. 2016.  Changes in the proteome of Langat-infected Ixodes scapularis ISE6 cells: metabolic pathways associated with flavivirus infection.  PLoS Negl. Trop. Dis., 10.2, e0004180. PMID: 26859745; PMCID: PMC4747643.

  • Gulia-Nuss, M., A. B. Nuss, J. M. Meyer, et al.  2016.  Genomic insights into the lxodes scapularis tick vector of Lyme disease. Nat. Commun. 7:10507.  PMID: 26856261 PMCID: PMC4748124.

2015

  • Jose, J., J. Tang, A. B. Taylor, T. S. Baker, and R. J. Kuhn.  2015.  Fluorescent protein-tagged Sindbis virus E2 glycoprotein allows single particle analysis of virus budding from live cells. Viruses 7:6182-6199.  PMID: 26633461; PMCID: PMC4690852.      

  • Yan, R., T. J. Edwards, L. M. Pankratz, R. J. Kuhn, J. K. Lanman, J. Liu, and W. Jiang.  2015.  A fast cross-validation method for alignment of electron tomography images based on Beer-Lambert law.  J. Struct. Biol., 192:297-306.  PMID: 26455556; PMCID: PMC4633358.

  • Yan, R., T. J. Edwards, L. M. Pankratz, R. J. Kuhn, J. K. Lanman, J. Liu, and W. Jiang.  2015.  Simultaneous determination of sample thickness, tilt, and electron mean fre path using tomographic tilt images based on Beer-Lambert law.  J. Struct. Biol., 192:287-296. PMID: 26433027; PMCID PMC4633387.

  • D’Aquila, T., D. Sirohi, J. M. Grabowski, V. E. Hedrick, L. N. Paul, A. S. Greenberg, R. J. Kuhn and K. K. Buhman. 2015. Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge. PLoS ONE, 10(5):e0126823. PMCID PMC4436333.

  • Kuhn, R. J., K. A. Dowd, C. B. Post and T. C. Pierson.  2015.  Shake, rattle, and roll: Impact of the dynamics of flavivirus particles on their interactions with the host.  Virology, May;479-480:508-517. PMID: 25835729.

  • Akey, D. L., W. C. Brown, J. Jose, R. J. Kuhn and J. L. Smith.  2015.  Structure-guided insights on the role of NS1 in flavivirus infection.  Bioessays, 37:489-494.  PMID: 25761098; PMCID: PMC4409125.

  • Liu, Y., J. Sheng, A. Fokine, G. Meng, F. Long, R. J. Kuhn, D. Kihara and M. G. Rossmann.  2015.  Virus structure.  Structure and inhibition of EV-D68, a virus that causes respiratory illness in children.  Science. 347:71-84.  PMID 25554786; PMCID: PMC4307789.

  • Zhang, X., J. Sheng, S. K. Austin, T. Hoornweg, J. Smit, R. Kuhn, M. Diamond and M. Rossmann.  2015.  Structure of acidic pH Dengue virus showing the fusogenic glycoprotein trimers. J. Virol. 89:743-750.  PMID:25355881; PMC4301137.

2014

  • Apte-Sengupta, S., D. Sirohi and R. J. Kuhn.  2014.  Coupling of replication and assembly in flaviviruses. Curr. Opin. Virol., 9:134-142. PMID:25462445; PMCID: PMC4268268.

  • Yun, S.I., B. H. Song, J. K. Kim, G. N. Yun, E. Y. Lee, L. Li, R. J. Kuhn, M. G. Rossmann, J. D. Morrey, and Y. M. Lee. 2014. A molecularly cloned, live-attenuated Japanese encephalitis vaccine SA14-14-2 virus: A conserved single amino acid in the ij hairpin of the viral E glycoprotein determines neurovirulence in mice. PLoS Pathog. 10(7): e1004290. PMID: 25077483; PMCID: PMC4117607.

  • Aggarwal, M., S. Dhindhwal, P. Kumar,  R. J.  Kuhn and S. Tomar.  2014.  Trans-protease activity and structural insights into the active form of the alphavirus capsid protease. J. Virol., 88:12242-12253.  PMID: 25100849; PMCID: PMC4248945.

  • Porta, J., J. Jose, J. T. Roehrig, C. D. Blair, R. J. Kuhn and M. G. Rossmann.  2014.  Locking and blocking the viral landscape of an alphavirus with neutralizing antibodies.  J. Virol., 88:9616-9623.  PMID: 24920796; PMCID: PMC4136364.

  • Langevin, S. A., R. A. Bowen, W. K. Reisen, C. C. Andrade, W. N. Ramey, P. D. Maharaj, M. Anishchenko, J. L. Kenney, N. K. Duggal, H. Romo, A. K. Bera, T. A. Sanders, A. Bosco-Lauth, J. L. Smith, R. Kuhn and A. C. Brault.  2014.  Host competence and helicase activity differences exhibited by West Nile viral variants expressing NS3-249 amino acid polymorphisms.  PLoS ONE 9(6): e100802. Doi:10.1371 

  • Schnettler, E., H. Tykalova, M. Watson, M. Sharma, M. Sterken, D. Obbard, S. Lewis, M. McFarlane, L. Bell-Sakyi, G. Barry, S. Weisheit, S. Best, R. Kuhn, G. Pijlman, M. Chase-Topping, E. A. Gould, L. Grubhoffer, J. Fazakerley, and A. Kohl.  2014.  Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses.  Nucl. Acids Res., 42(14):9436-9446.  PMID: 25053841.

  • Dowd, K. A., S. Mukherjee, R. J. Kuhn and T. C. Pierson.  2014.  Combined effects of the structural heterogeneity and dynamics of flaviviruses on antibody recognition.  J. Virol. 88:11726-11737. PMID: 25078693; PMCID: PMC4178732.

  • Yu, G., F. Vago, D. Zhang, J. E. Snyder, R. Yan, C. Zhang, C. Benjamin, X. Jiang, R. J. Kuhn, P. Serwer, D. H. Thompson and W. Jiang. 2014.  Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies.  J. Struct.Biol. 187:1-9.  PMID: 24780590; PMCID PMC4060259.

  • Junjhon, J., J. G. Pennington, T. J. Edwards, R. Perera, J. Lanman and R. J. Kuhn.  2014.  Ultrastructural characterization and three-dimensional architecture of replication sites in dengue virus-infected mosquito cells.  J. Virol. 88:4687-4697. PMID: 24522909; PMCID PMC3993787.

  • Plevka, P., P.-Y.- Lim, R. Perera, J. Cardosa, A. Suksatu, R. J. Kuhn, and M. G. Rossmann.  2014.  Neutralizing antibodies can initiate genome release from human enterovirus 71.  Proc. Natl. Acad. Sci., 111:2134-2139. PMID 24469789; PMCID: PMC3926013

  • Akey, D. L., C. W. Brown, S. Dutta, Konwerski, J. Jose, T. J. Jurkiw, J. DelProposto, C. M. Ogata, G. Skiniotis, R. J. Kuhn and J. L. Smith.  2014.  Flavivirus NS1 structures reveal surfaces for association with membranes and the immune system. Science 343:881-885.  PMID: 24505133

2013

  • Dai, H.-S., Z. Liu, W. Jiang, and R. Kuhn.  2013.  Directed evolution of a virus exclusively utilizing human epidermal growth factor receptor as the entry receptor.  J. Virol., 87:11231-11243. PMID: 23926357; PMCID: PMC3807306.

  • Snyder, J.E., K. A. Kulcsar, K. L. Schultz, C. P. Riley, J. T Neary, S. Marr, J. Jose, D. E. Griffin, and R. J. Kuhn.  2013.  Functional characterization of the Alphavirus TF Protein. J. Virol. 87:8511-8523. PMID: 23720714; PMCID: PMC3719798.

  • Keating, J. A., D. Bhattacharya, P-Y. Lim, S. Falk, B. Weisblum, K. A. Bernard, M. Sharma, R. J. Kuhn and R. Striker.  2013.  West Nile virus methyltransferase domain interacts with protein kinase G. Virol. J., 10:242.  PMID: 23876037; PMCID: PMC3725163.

  • Zhang, W., B. Kaufmann, P. R. Chipman, R. J. Kuhn, and M. G. Rossmann.  2013.  Membrane curvature in flaviviruses.  J. Struct. Biol., 183:86-94. PMID: 23602814 PMCID: PMCID: PMC4091808.                      

  • Catanese, M.T., K. Uryu, M. Kopp, T. J. Edwards, L. Andrus, W. J. Rice, M. Silvestry, R. J. Kuhn, and C. M. Rice.  2013.  Ultrastructural analysis of hepatitis C virus particles.  Proc. Natl. Acad. Sci. USA, 110:9505-9510. PMCID: PMC3677472; PMID: 23690609.Zhang, X., J. Sheng, P. Plevka, R. J. Kuhn, M. S. Diamond, and M. G. Rossmann.  2013.  Dengue structure differs at the temperatures of its human and mosquito hosts.  PNAS, 110:6795-6799. PMID 23569243; PMCID: PMC3637732

  • Kuhn, R. J.  2013.  Chapter 22: Togaviridae. pp.629-650. In D. M. Knipe and P. M. Howley (eds), Fields Virology, 6th Ed., Lippincott Williams & Wilkins.

  • Plevka, P., R. Perera, M. L. Yap, J. Cardosa, R. J. Kuhn, M. G. Rossmann.  2013.  Structure of human enterovirus 71 in complex with a capsid binding inhibitor.  Proc. Natl. Acad. Sci. USA, 110:5463-5467.  PMID: 23509286; PMCID: PMC3619292.

2012

  • Snyder, J. E., C. J. Berrios, T. J. Edwards, J. Jose, R. Perera, and R. J. Kuhn.  2012.  Probing the early temporal and spatial interaction of the Sindbis virus capsid and E2 proteins with reverse genetics. J. Virol., 86:12372-12383. PMID: 22951842; PMCID: PMC3486501.

  • Aggarwal, M., S. Tapas, Preeti, A. Siwach, P. Kumar, R. J. Kuhn, and S. Tomar  2012.  Crystal structure of aura virus capsid protease and its complex with dioxane: new insights into capsid-glycoprotein molecular contacts.  PLoS ONE 7(12): e51288.  PMCID: PMC3522669

  • Plevka, P., R. Perera, J. Cardosa, R. J. Kuhn, and M. G. Rossmann.  2012.  Structure determination of enterovirus 71.  Acta. Crystallogr. D Biol. Crystallogr. 68:1217-1222.  PMID: 22948923; PMCID: PMC3489104.

  • Battisti, A. J., J. D. Yoder, P. Plevka, D. C. Winkler, V. M. Prasad, R. J. Kuhn, T. K. Frey, A. C. Steven, and M. G. Rossmann.  2012.  Cryo-electron tomography of rubella virus.  J. Virol., 86:11078-11085. PMID: 22855483; PMCID: PMC3457135.

  • Botting, C. and R. J. Kuhn.  2012.  Novel approaches to flavivirus drug discovery.  Expert. Opin. Drug Discov. 7:417-428.  PMID: 22439769.

  • Perera, R., C. Riley, G. Isaac, A. Hopf-Jannasch, R. J. Moore, K. W. Weitz, L. Pasa-Tolic, T. O. Metz, J. Adamec and R. J. Kuhn. 2012.  Dengue virus infection perturbs lipid homeostasis in infected mosquito cells. PLoS Pathog. 8(3): e1002584. PMID: 22457619; PMCID: PMC3310792.

  • Plevka, P., R. Perera, J. Cardosa, R. J. Kuhn and M. G. Rossmann.  2012. Crystal structure of human Enterovirus 71. Science 8:336:1274.PMID: 22383808. PMCID: PMC3448362.

  • Pierson, T. C. and R. J. Kuhn.  2012.  Capturing a virus while it catches its breath. Structure 20:200-202. PMID: 22325768; PMCID: PMC3733089.

  • Jose, J., L. Przybyla, T. J. Edwards, R. Perera, J. W.  Burgner, and R. J. Kuhn. 2012.  Interactions of the cytoplasmic domain of Sindbis virus E2 with nucleocapsid cores promote alphavirus budding. J. Virol., 86:2585-2599.  PMID: 22190727; PMCID: PMC3302261.

2011

  • Tang, J., J. Jose, P. Chipman, W. Zhang, R. J. Kuhn, and T. S. Baker.  2011.  Molecular links between the E2 envelope glycoprotein and nucleocapsid core in Sindbis virus J. Mol. Biol., 414:442-459.  PMID:22001018.

  • Aggarwal, M., S. Dhindwal, S. Pratap, R. J. Kuhn, P. Kumar and S. Tomar.  2011. Crystallization, high-resolution data collection and preliminary crystallographic analysis of Aura virus capsid protease and its complex with dioxane.  Acta. Cryst. F67:1394-1398.  PMID:22102240; PMCID PMC321245.

  • Khadka, S., A. D., Vangeloff, C. Zhang, P. Siddavatam, N. S. Heaton, L. Wang, R. Sengupta, S. Sahasrabudhe, G. Randall, M. Gribskov, R. J. Kuhn, R. Perera, and D. J. LaCount.  2011.  A physical interaction network of dengue virus and human proteins.  Mol. Cell Proteomics 10(12)M111.012187-16. PMID: 21911577.

  • Agbandje-McKenna, M. and Kuhn, R. 2011. Current opinions in virology: structural virology.  Current Opinion in Virology 1:81-83.

  • Kuhn, R. J.  2011.  Chapter 2.  Flavivirus virion structure, pp.9-20.  In Molecular Virology and Control of Flaviviruses, ed. P.-Y. Shi, Caister Academic Press.

  • Tomar, S., M. Narwal, E. Harms, J. L. Smith and R. J. Kuhn. 2011.  Heterologous production, purification and characterization of enzymatically active Sindbis virus nonstructural protein nsP1. Protein Expr. Purif. 79:277-284.  PMID:21693190.

  • Plevka, P., A. J. Battisti, J. Junjhon, D. C. Winkler, H. A. Holdaway, P. Keelapang, N. Sittisombut, R. J. Kuhn, A. C. Steven, and M. G. Rossmann.  2011.  Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres. EMBO Rep. 12:602-606. Doi:10.1038/embor.2011.75; PMID: 21566648; PMCID: PMC3128282.

  • Mayhoub A. S., M. Khaliq, C. Botting, Z. Li, R. J. Kuhn, and M. Cushman. 2011. An investigation of phenylthiazole antiflaviviral agents. Bioorg. Med. Chem., 19(12):3845-54.

  • Plevka, P., A. J. Battisti, J. Junjhon, D. C. Winkler, H. A. Holdaway, P. Keelapang, N. Sittisombut, R. J. Kuhn, A. C. Steven, and M. G. Rossmann. 2011. Maturation of flaviruses starts from one or more icosahedrally independent nucleation centres. EMBO Rep., 12(6):602-6.

  • Snyder, J.E., O. Azizgolshani, B. Wu, Y. He, A. C. Lee, J. Jose, D. M. Suter, C. M. Knobler, W. M. Gelbart, and R. J. Kuhn. 2011. Rescue of infectious particles from pre-assembled alphavirus nucleocapsid cores. J. Virol., 85(12):5773-81.

  • Mayhoub, A. S., M. Khaliq, R. J. Kuhn and M. Cushman. 2011. Design, synthesis, and biological evaluation of thiazoles targeting flavivirus envelope proteins. J. Med. Chem., 54:1704-1714.

2010

  • Kuhn, R. J. 2010. Chapter 2. Flavivirus virion structure. In Molecular Virology and Control of Flaviviruses, Horizon Scientific Press, in press.

  • Gualtieri, E.J., F. Guo, D. J. Kissick, J. Jose, R. J. Kuhn, W. Jiang and G. J. Simpson. 2010. Detection of membrane protein two-dimensional crystals in living cells. Biophys. J., 100:207-214.

  • Kaufmann, B., M. R. Vogt, J. Goudsmit, H. A. Holdaway, A. A. Aksyuk, , P. R. Chipman, R. J. Kuhn, M. S. Diamond and M. G. Rossmann. 2010. Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354. Proc. Natl. Acad. Sci., 107:18950-18955.

  • Li, L., J. Jose, Y. Xiang, R. J. Kuhn and M. G. Rossmann. 2010. Structural changes of envelope proteins during alphavirus fusion. Nature, 468:705-708.

  • Heaton, N. S., R. Perera, K. L. Berger, S. Khadka, D. J. LaCount, R. J. Kuhn and G. Randall. 2010. Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis. Proc. Natl. Acad. Sci., 107:17345-17350.

  • Junjhon, J., T. J. Edwards, U. Utaipat, V. D. Bowman, H. A. Holdaway, W. Zhang, P. Keelapang, C. Puttikhunt, R. Perera, P. R. Chipman, W. Kasinrerk. P. Malasit, R. J. Kuhn and N. Sittisombut. 2010. Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles. J. Virol., 84:8353-8358.

  • Kaufmann, B., P. Plevka, R. Kuhn and M. Rossmann. 2010. Crystallization and preliminary X-ray diffraction analysis of West Nile virus. Acta Cryst. F66:558-562.

  • Kuhn, R. J. 2010. Chapter 13: Togaviruses. In N. A. Acheson (ed.), Fundamentals of Molecular Virology, John Wiley & Sons, Inc. in press.

  • Kuhn, R. J. 2010. Chapter 12: Flaviviruses. In N. A. Acheson (ed.), Fundamentals of Molecular Virology, John Wiley & Sons, Inc. in press.

  • Mayuri, E. LaBauve, and R. J. Kuhn. 2010. Chapter 13. Novel therapeutic approaches for Dengue disease. In: Frontiers in Dengue Virus Research, eds. K. A. Hanley and S. C. Weaver, Caister Academic Press, pages 239-263.

2009

  • Kaufmann, B., P. R. Chipman, H. A. Holdaway, S. Johnson, D. H. Fremont, R. J. Kuhn, M. S. Diamond and M. G. Rossmann. 2009. Capturing a flavivirus pre-fusion intermediate. PLoS Pathog. 5(11)e1000672.

  • Yu, I.-E., H. A. Holdaway, P. R. Chipman, R. J. Kuhn, M. G. Rossmann, and J. Chen. 2009. Association of the pr peptides with dengue virus at acidic pH blocks membrane fusion. J. Virol. 83:12101-12107.

  • Patkar, C. G., M. Larsen, M. Owston, J. L. Smith and R. J. Kuhn. 2009. Identification of inhibitors of yellow fever virus replication using a replicon-based high-throughput assay. Antimicrob. Agents Chemother. 53:4103-4014.

  • Jose, J., J. E. Snyder, and R. J. Kuhn. 2009. A structural and functional perspective of alphavirus replication and assembly. Future Microbiol. 4:837-856.

  • Cherrier, M.V., B. Kaufmann, G.E. Nybakken, S-M. Lok, J.T. Warren, B.R. Chen, C. A. Nelson, V.A. Kostyuchenko, H.A. Holdaway, P.R. Chipman, R.J. Kuhn, M.S. Diamond, M.G. Rossmann and D.H. Fremont. 2009. Structural basis for the preferential recognition of immature flaviviruses by a fusion-loop antibody. EMBO J. 28:3269-3276.

  • Bhattacharya, D., Mayuri, S.M. Best, R. Perera, R.J. Kuhn, R. Striker. 2009. Protein kinase G phosphorylates mosquito-borne flavivirus NS5. J. Virol. 83: 9195-9205.

2008

  • Chang, W. S., Shang, H., Perera, R. M., Lok, S. M., Sedlak, D., Kuhn, R. J., Lee, G. U. 2008. Rapid detection of dengue virus in serum using magnetic separation and fluorescence detection. Analyst. 133: 233-240. view article

  • Perera, R., Khaliq, M., Kuhn, R. J. 2008. Closing the door on flaviviruses: Entry as a target for antiviral drug design. Antiviral Res. 80: 11-22.

  • Yu, I. M., Zhang, W., Holdaway, H. A., Li, L., Kostyuchenko, V. A., Chipman, P. R., Kuhn, R. J., Rossmann, M. G., Chen, J. 2008. Structure of the immature dengue virus at low pH primes proteolytic maturation. Science. 319: 1834-1837.

  • Lok, S. M., Kostyuchenko, V., Nybakken, G. E., Holdaway, H. A., Battisti, A. J., Sukupolvi-Petty, S., Sedlak, D., Fremont, D. H., Chipman, P. R., Roehrig, J. T., Diamond, M. S., Kuhn, R. J., Rossmann, M. G. 2008. Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins. Nat Struct Mol Biol. 15: 312-317.

  • Patkar, C. G., Kuhn, R. J. 2008. Yellow fever virus NS3 plays an essential role in virus assembly independent of its known enzymatic functions. J Virol. 82: 3342-3352.

  • Warrier, R., Linger, B. R., Golden, B. L., Kuhn, R. J. 2008. Role of Sindbis Virus Capsid Protein Region Ii in Nucleocapsid Core Assembly and Encapsidation of Genomic Rna. J Virol. 82: 4461-4470.

  • Mayuri, Geders, T. W., Smith, J. L., Kuhn, R. J. 2008. Role for conserved residues of sindbis virus nonstructural protein 2 methyltransferase-like domain in regulation of minus-strand synthesis and development of cytopathic infection. J Virol. 82: 7284-7297.

  • Li, L., Lok, S. M., Yu, I. M., Zhang, Y., Kuhn, R. J., Chen, J., Rossmann, M. G. 2008. The flavivirus precursor membrane-envelope protein complex: structure and maturation. Science. 319: 1830-1834.

  • Perera, R., Kuhn, R. J. 2008. Structural proteomics of dengue virus. Curr. Opin. Microbiol. 11: 369-377.

2007

  • Bera, A. K., Kuhn, R. J., Smith, J. L. 2007. Functional characterization of cis and trans activity of the Flavivirus NS2B-NS3 protease. J Biol Chem. 282: 12883-12892.

  • Boudko, S. P., Kuhn, R. J., Rossmann, M. G. 2007. The coiled-coil domain structure of the Sin Nombre virus nucleocapsid protein. J Mol Biol. 366: 1538-1544.

  • Kim, H. Y., Kuhn, R. J., Patkar, C., Warrier, R., Cushman, M. 2007. Synthesis of dioxane-based antiviral agents and evaluation of their biological activities as inhibitors of Sindbis virus replication. Bioorg Med Chem. 15: 2667-2679.

  • Navaratnarajah, C. K., Kuhn, R. J. 2007. Functional characterization of the Sindbis virus E2 glycoprotein by transposon linker-insertion mutagenesis. Virology. 363: 134-147.

  • Patkar, C. G., Jones, C. T., Chang, Y. H., Warrier, R., Kuhn, R. J. 2007. Functional requirements of the yellow fever virus capsid protein. J Virol. 81: 6471-6481.

  • van der Schaar, H. M., Rust, M. J., Waarts, B. L., van der Ende-Metselaar, H., Kuhn, R. J., Wilschut, J., Zhuang, X., Smit, J. M. 2007. Characterization of the early events in dengue virus cell entry by biochemical assays and single-virus tracking. J Virol. 81: 12019-12028.

  • Zhang, Y., Kaufmann, B., Chipman, P. R., Kuhn, R. J., Rossmann, M. G. 2007. Structure of immature West Nile virus. J Virol. 81: 6141-6145.

2006

  • Hong, E. M., Perera, R., Kuhn, R. J. 2006. Alphavirus capsid protein helix I controls a checkpoint in nucleocapsid core assembly. J Virol. 80: 8848-8855.

  • Kaufmann, B., Nybakken, G. E., Chipman, P. R., Zhang, W., Diamond, M. S., Fremont, D. H., Kuhn, R. J., Rossmann, M. G. 2006. West Nile virus in complex with the Fab fragment of a neutralizing monoclonal antibody. Proc Natl Acad Sci U S A. 103: 12400-12404.

  • Mukhopadhyay, S., Zhang, W., Gabler, S., Chipman, P. R., Strauss, E. G., Strauss, J. H., Baker, T. S., Kuhn, R. J., Rossmann, M. G. 2006. Mapping the structure and function of the E1 and E2 glycoproteins in alphaviruses. Structure. 14: 63-73.

  • Patkar, C. G., Kuhn, R. J. 2006. Development of novel antivirals against flaviviruses. Novartis Found Symp. 277: 41-52 discussion 52-46, 71-43, 251-253.

  • Pokidysheva, E., Zhang, Y., Battisti, A. J., Bator-Kelly, C. M., Chipman, P. R., Xiao, C., Gregorio, G. G., Hendrickson, W. A., Kuhn, R. J., Rossmann, M. G. 2006. Cryo-EM reconstruction of dengue virus in complex with the carbohydrate recognition domain of DC-SIGN. Cell. 124: 485-493.

  • Tomar, S., Hardy, R. W., Smith, J. L., Kuhn, R. J. 2006. Catalytic core of alphavirus nonstructural protein nsP4 possesses terminal adenylyltransferase activity. J Virol. 80: 9962-9969.

2005

  • Jones, C. T., Patkar, C. G., Kuhn, R. J. 2005. Construction and applications of yellow fever virus replicons. Virology. 331: 247-259.

  • Kim, H. Y., C. Patkar, R. Warrier, R. Kuhn and M. Cushman. 2005. Design, synthesis, and evaluation of dioxane-based antiviral agents targeted against the Sindbis virus capsid protein. Bioorg Med Chem Lett. 2005 Jul 1;15(13):3207-11.

  • Wilkinson, T. A., T. L. Tellinghuisen, R. J. Kuhn and C. B. Post. 2005. Association of Sindbis Virus Capsid Protein with Phospholipid Membranes and the E2 Glycoprotein: Implications for Alphavirus Assembly. Biochemistry. 2005 Mar 1;44(8):2800-10.

  • Mukhopadhyay, S., R. J. Kuhn and M. G. Rossmann. 2005. A structural perspective of the flavivirus lifecycle. Nat Rev Microbiol. 2005 Jan;3(1):13-22. Review.

  • Zhang, W., M. Heil, R. J. Kuhn and T. S. Baker. 2005. Heparin binding sites on Ross River Virus revealed by electron cryo-microscopy. Virology 332:511-518.

  • Jones, C. T., C. G. Patkar and R. J. Kuhn. 2005. Construction and applications of yellow fever virus replicons. Virology 331:247-259.

  • Kuhn, R. J. and M. G. Rossmann. 2005. Structure and assembly of icosahedral enveloped RNA viruses. In: Advances in Virus Research. Roy, P. (ed.) Academic Press.

2004

  • Choi, K. H., J. M. Groarke, D. C. Young, M. G. Rossmann, D. C. Pevear, R. J. Kuhn and J. L. Smith. 2004. Design, expression, and purification of a Flaviviridae polymerase using a high-throughput approach to facilitate crystal structure determination. Protein Science 13:2685-2692.

  • Choi, K. H., J. M. Groarke, D. C. Young, R. J. Kuhn, J. L. Smith, D. C. Pevear and M. G. Rossmann.. 2004. The structure of the RNA-dependent RNA polymerase from bovine viral diarrhea virus establishes the role of GTP in de novo initiation. Proc. Natl. Acad. Sci. USA 101:4425-4430.

  • Zhang, Y., W. Zhang, S. Ogata, D. Clements, J. H. Strauss, T. S. Baker, R. J. Kuhn and M. G. Rossmann. 2004. Conformational changes of the flavivirus E glycoprotein. Structure 12:1607-1618.

  • Ma, L., C. T. Jones, T. D. Groesch, R. J. Kuhn and C. B. Post 2004. Solution structure of dengue virus capsid protein reveals another fold. Proc. Natl. Acad. Sci. USA 101:3414-3419.

  • Linger, B. R., L. Kunovska, R. J. Kuhn and B. L. Golden. 2004 Sindbis virus nucleocapsid assembly: RNA folding promotes capsid protein dimerization. RNA 10:128-138.

  • Menon, E. L., R. Perera, M. Navarro, R. J. Kuhn and H. Morrison. 2004. Phototoxicity against tumor cells and sindbis virus by an octahedral rhodium bisbipyridyl complex and evidence for the genome as a target in viral photoinactivation. Inorg. Chem. 43:5373-5381.

2003

  • Kuhn, R. J. and J. H. Strauss. 2003. Enveloped Viruses. Pp. 363-377. In: Virus Structures. Chiu, W. and Johnson, J. E. (eds.) Advances in Protein Chemistry (Vol. 64). Academic Press.

  • Mukhopadhyay, S., B-S. Kim, P. R. Chipman, M. G. Rossmann and R. J. Kuhn. 2003. Structure of West Nile virus. Science 302:248.

  • Menon, E. L., R. Perera, R. J. Kuhn and H. Morrison. 2003 Reactive oxygen species formation by UVA irradiation of urocanic acid and the role of trace metals in this chemistry Photochemistry and Photobiology 78:567-575.

  • Zhang, W., P. R. Chipman, J. Corver, P. R. Johnson, Y. Zhang, S. Mukhopadhyay, T. S. Baker, J. H. Strauss, M. G. Rossmann and R. J. Kuhn. 2003 Visualization of membrane protein domains by cryo-electron microscopy of dengue virus Nat. Struct. Biol. 10:907-912.

  • Perera, R., C. Navaratnarajah and R. J. Kuhn. 2003. A heterologous coiled coil can substitute for helix I of the Sindbis virus capsid protein J. Virol: 77:8345-8353.

  • Zhang, Y., J. Corver, P. R. Chipman, W. Zhang, S. V. Pletnev, D. Sedlak, T. S. Baker, J. H. Strauss, R. J. Kuhn and M. G. Rossmann. 2003. Structures of immature flavivirus particles EMBO J. 22:2604-2613.

  • Jones, C. T., L. Ma, J. W. Burgner, T. D. Groesch, C. B. Post, R. J. Kuhn. 2003. Flavivirus capsid protein is a dimeric alpha-helical protein. J. Virol: 77:7143-7149.

  • He, Y., S. Mueller, P. R. Chipman, C. M. Bator, X. Peng, V. D. Bowman, S. Mukhopadyay, E. Wimmer, R. J. Kuhn, and M. G. Rossmann. 2003. Complexes of poliovirus serotypes with their common cellular receptor, PVR. J. Virol: 77:4827-4835.

2002

  • Zhang, W., S. Mukhopadhyay, S. V. Pletnev, T. S. Baker, R. J. Kuhn and M. G. Rossmann. 2002. Placement of the structural proteins in Sindbis virus. J. Virol. 76:11645-11658.

  • Mukhopadhyay, S., P. R. Chipman, E. M. Hong, R. J. Kuhn and M. G. Rossmann. 2002. In vitro assembled alphavirus core-like particles maintain a structure similar to nucleocapsid cores in mature virus. J. Virol: 76:11128-11132.

  • Rossmann, M. G., Y. He and R. J. Kuhn. 2002. Picornavirus-receptor interactions. Trends in Microbiology 10:324-331.

  • He, Y., F. Lin, P. R. Chipman, C. M. Bator, T. S. Baker, M. Shoham, R. J. Kuhn, M. E. Medof and M. G. Rossmann. 2002. Structure of decay-accelerating factor bound to echovirus 7: a virus-receptor complex. Proc. Natl. Acad. Sci. USA 99:10325-10329.

  • Zhang, W., B. R. Fisher, N. H. Olson, J. H. Strauss, R. J. Kuhn. and T. S. Baker. 2002. Aura virus structure suggests that the T_4 organization is a fundamental property of viral structural proteins. J. Virol: 76:7239-7246.

  • Kuhn, R. J. and M. G. Rossmann. 2002. Interaction of major group rhinoviruses with their cellular receptor, ICAM-1 pp. 85-91. In: Molecular Biology of Picornaviruses, Semler, B. L. and Wimmer, E. (eds.) ASM Press.

  • Kuhn, R. J., W. Zhang, M. G. Rossmann, S. V. Pletnev, J. Corver, E. Lenches, C. T. Jones, S. Mukhopadhyay P. R. Chipman, E. G. Strauss, T. S. Baker and J. H. Strauss. 2002. Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell 108:717-725.

2001

  • Rieder, E., A. E. Gorbalenya, C. Xiao, Y. He, T. S. Baker, R. J. Kuhn, M. G. Rossmann and E. Wimmer. 2001. Will the polio niche remain vacant? In: Progress in Polio Eradication: Vaccine Strategies for the End Game. Brown, F. (ed.) Dev. Biol. 105:111-112.

  • He, Y., P. R. Chipman, J. Howitt, C. M. Bator, M. A. Whitt, T. S. Baker, R. J. Kuhn, C. W. Anderson, P. Freimuth, P. and M. G. Rossmann. 2001. Interaction of coxsackievirus B3 with the full-length coxsackievirus-adenovirus receptor. Nature Struct Biol. 8:874-878.

  • Heil, M., A. Albee, J. H. Strauss and R. J. Kuhn. 2001. An amino acid substitution in the coding region of the E2 glycoprotein adapts Ross River Virus to utilize Heparan Sulfate as an attachment moiety J. Virol. 75:6303-6309.

  • Pletnev, S. V., W. Zhang, S. Mukhopadhyay, B. R. Fisher, R. Hernandez, D. T. Brown, T. S. Baker, M. G. Rossmann and R. J. Kuhn. 2001. Locations of carbohydrate sites on alphavirus glycoproteins show that E1 forms an icosahedral scaffold Cell 105:127-136.

  • Tellinghuisen, T. L., R. Perera and R. J. Kuhn. 2001. Genetic and biochemical studies on the assembly of an enveloped virus. In: Genetic Engineering, Principles and Methods, Vol. 23, Setlow, J. K. (ed.) pp. 83-112.

  • Tellinghuisen, T. L., R. Perera and R. J. Kuhn. 2001. In vitro assembly of Sindbis nucleocapsid-like particles from cross-linked dimers of truncated and mutant capsid proteins J. Virol. 75:2810-2817.

  • Sharkey, C. M., C. L. North, R. J. Kuhn and D. A. Sanders. 2001. Ross River virus glycoprotein-pseudotyped retroviruses and stable cell lines for their production J. Virol. 75:2653-2659.

  • Xiao, C., C. M. Bator, V. D. Bowman, E. Rieder, Y. He, B. Hebert, J. Bella, T. S. Baker, E. Wimmer, R. J. Kuhn and M. G. Rossmann. 2001. Interaction of coxsackievirus A21 with its cellular receptor, ICAM-1 J. Virol. 75:2444-2451.

  • Perera, R., K. E. Owen, T. L. Tellinghuisen, A. E. Gorbalenya and R. J. Kuhn. 2001. Alphavirus nucleocapsid protein contains a putative coiled coil α-helix important for core assembly J. Virol. 75:1-10.

2000 and earlier

  • Rossmann, M. G., J. Bella, P. R. Kolatkar, Y. He, E. Wimmer, R. J. Kuhn and T. S. Baker. 2000. Cell recognition and entry by rhino- and enteroviruses Virology 269:239-247.

  • Tellinghuisen, T. L. and R. J. Kuhn. 2000. Nucleic acid-dependent crosslinking of the nucleocapsid protein of Sindbis virus J. Virol. 74:4302-4309.

  • Houghtaling, M. A., R. Perera, K. E. Owen, S. Wagner, R. J. Kuhn and H. Morrison. 2000. Photobiological properties of positively charged methylene violet analogs. Photochemistry & Photobiology. 71(1):20-28.

  • He, Y., V. D. Bowman, S. Mueller, C. M. Bator, J. Bella, X. Peng, T. S. Baker, E. Wimmer, R. J. Kuhn and M. G. Rossmann. 2000. Interaction of the poliovirus receptor with poliovirus Proc. Natl. Acad. Sci. USA 97:79-84.

  • Tellinghuisen, T. L., A. E. Hamburger, B. R. Fisher, R. Ostendorp and R. J. Kuhn. 1999. In vitro assembly of alphavirus cores by using nucleocapsid protein expressed in Escherichia coli. J. Virol. 73:5309-5319.

  • Lee, S., R. J. Kuhn and M. G. Rossmann. 1998. Probing the potential glycoprotein binding site of Sindbis virus capsid protein with dioxane and model building. Proteins: Structure, Function, and Genetics 33:311-317.

  • Kuhn, R. J. 1997. Identification and biology of cellular receptors for the CVB group, pp. 209-226. In The Coxsackie B Viruses, Tracy, S., Chapman, N. M. and Mahy, B. W. J. (eds.), Springer Verlag, Vienna, Austria.

  • Owen, K. E. and R. J. Kuhn. 1997. Alphavirus budding is dependent on the interaction between the nucleocapsid and hydrophobic amino acids on the cytoplasmic domain of the E2 envelope glycoprotein. Virology 230:187-196.

  • Zhao, R., D. C. Pevear, M. J. Kremer, V. L. Giranda, J. A. Kofron, R. J. Kuhn and M. G. Rossmann. 1996. Human rhinovirus 3 at 3.0 resolution. Structure 4:1205-1220.

  • Kuhn, R. J., D. E. Griffin, K. E. Owen, H. G. M. Niesters and J. H. Strauss. 1996. Chimeric Sindbis-Ross River viruses to study interactions between alphavirus nonstructural and structural regions. J. Virol. 70:7900-7909.

  • Choi, H-K., S. Lee, Y-P. Zhang, B. R. McKinney, G. Wengler, M. G. Rossmann and R. J. Kuhn. 1996. Structural analysis of Sindbis virus capsid mutants involving assembly and catalysis. J. Mol. Biol. 262:151-167.

  • Lee, S., K. E. Owen, H-K. Choi, H. Lee, G. Lu, G. Wengler, D. T. Brown, M. G. Rossmann and R. J. Kuhn. 1996. Identification of a protein binding site on the surface of the alphavirus nucleocapsid protein and its implication in virus assembly. Structure 4:531-541.

  • Owen, K. E. and R. J. Kuhn. 1996. Identification of a region in the Sindbis virus nucleocapsid protein that is involved in specificity of RNA encapsidation. J. Virol. 70:2757-2763.

  • Kuhn, R. J. and M. G. Rossmann. 1995. When it's better to lie low, In News and Views, Nature 375:275-276.

  • Smith, T. J., R. H. Cheng, N. H. Olson, P. Peterson, E. Chase, R. J. Kuhn and T. S. Baker. 1995. Putative receptor binding sites on alphaviruses as visualized by cryo-electron microscopy. Proc. Natl. Acad. Sci. USA 92:10648-10652.

  • Strauss, J. H., E. G. Strauss and R. J. Kuhn. 1995. Budding of alphaviruses. Trends in Microbiology 3:346-350.

  • Cheng, R. H., R. J. Kuhn, N. H. Olson, M. G. Rossmann, H-K. Choi, T. J. Smith and T. S. Baker. 1995. Nucleocapsid and Glycoprotein Organization in an Enveloped Virus. Cell 80:621-630.

  • Strauss, J.H., T. Rmenapf, R. C. Weir, R. J. Kuhn, K. Wang and E. G. Strauss. 1994. Cellular receptors for alphaviruses, pp. 141-163 In Cellular Receptors for Animal Viruses, Wimmer, E. ed., Cold Spring Harbor Press.

  • Lopez, S., J. S. Yao, R. J. Kuhn, E. G. Strauss and J. H. Strauss. 1994. Nucleocapsid-glycoprotein interactions required for assembly of alphaviruses. J. Virol. 68:1316-1323.

  • Strauss, J.H., K. Wang, A.L. Schmaljohn, R. J. Kuhn and E. G. Strauss. 1994. Host-cell receptors for Sindbis virus, pp. 473-484. In Positive Strand RNA Viruses, Brinton, M.A. and Rueckert, R.R. (eds.), Springer Verlag, Vienna, Austria.

  • Cao, X., R. J. Kuhn and E. Wimmer. 1993. Replication of poliovirus RNA containing two VPg genes leads to deletion of one VPg by recombination. J. Virol. 67:5572-5578.

  • Tucker, P. C., E. G. Strauss, R. J. Kuhn, J. H. Strauss and D. E. Griffin. 1993. Viral determinants of age-dependent virulence of Sindbis virus for mice. J. Virol. 67:4605-4610.

  • Kuhn, R. J., D. E. Griffin, H. Zhang, H. G. M. Niesters and J. H. Strauss. 1992. Attenuation of Sindbis virus neurovirulence using defined mutations in the nontranslated regions of the genome RNA. J. Virol. 66:7121-7127.

  • Wang, K. S., R. J. Kuhn, E. G. Strauss, S. Ou and J. H. Strauss. 1992. High-affinity laminin receptor is a receptor for Sindbis virus in mammalian cells. J. Virol. 66:4992-5001.

  • Wang, K. S., A. L. Schmaljohn, R. J. Kuhn and J. H. Strauss. 1991. Antiidiotypic antibodies as probes for the Sindbis virus receptor. Virology 181:694-702.

  • Kuhn, R. J., H. G. M. Niesters, Z. Hong and J. H. Strauss. 1991. Infectious RNA transcripts from Ross River virus cDNA clones and the construction and characterization of defined chimeras with Sindbis virus. Virology 182:430-441.

  • deGroot, R. J., T. Rmenapf, R. J. Kuhn, E. G. Strauss and J. H. Strauss. 1991. Sindbis virus RNA polymerase is degraded by the N-end rule pathway. Proc. Natl. Acad. Sci. USA 88:8967-8971.

  • Reuer, Q., R. J. Kuhn and E. Wimmer. 1990. Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg. J. Virol. 64:2967-2975.

  • Strauss, J. H., R. J. Kuhn, H. G. M. Niesters and E. G. Strauss. 1990. Functions of the 5'-terminal and 3'-terminal sequences of the Sindbis virus genome in replication, pp. 61-66. In Brinton, M. A. and F. X. Heinz (eds.), New Aspects of Positive-Strand RNA Viruses. ASM Publications, Washington, DC.

  • Kuhn, R. J., Z. Hong and J. H. Strauss. 1990. Mutagenesis of the 3' nontranslated region of Sindbis virus RNA. J. Virol. 64:1465-1476.

  • Bradley, J., M. G. Murray, R. J. Kuhn, H. Tada, X. F. Yang, C. Mirzayan and E. Wimmer. 1989. Use of mutagenesis cartridges in molecular genetic analyses of poliovirus: mutations in the genome-linked protein, VPg and in neutralization antigenic site I, pp. 3-25. In Semler, B. L. and E.Ehrenfeld (eds.), Molecular Aspects of Picornavirus Infection and Detection. ASM Publications, Washington, DC.

  • Semler, B. L., R. J. Kuhn and E. Wimmer. 1988. Replication of the poliovirus genome, pp. 23-48. In Holland, J. J., P. Alhquist, D. Domingo (eds.), RNA Genetics. CRC Press.

  • Murray, M. G., R. J. Kuhn and E. Wimmer. 1988. Poliovirus Type 1 and 3 neutralizing antibodies in rabbits, pp. 197-204. In Lerner, R. A., R. M. Chanock, F. Brown, and H. Ginsberg (eds.), Vaccines 88. Cold Spring Harbor Lab, Cold Spring Harbor, NY.

  • Murray, M. G., R. J. Kuhn, M. Arita, N. Kawamura, A. Nomoto and E. Wimmer. 1988. Poliovirus Type 1/Type 3 antigenic hybrid virus constructed in vitro elicits Type 1 and Type 3 neutralizing antibodies in rabbits and monkeys. Proc. Natl. Acad. Sci. USA 85:3203-3207.

  • Kuhn, R. J., H. Tada, M. F. Ypma-Wong, B. L. Semler and E. Wimmer. 1988. Mutational analysis of the genome-linked protein of poliovirus. J. Virol. 62:4207-4215.

  • Kuhn, R. J., H. Tada, M. F. Ypma-Wong, J. J. Dunn, B. L. Semler and E. Wimmer. 1988. Construction of a "mutagenesis cartridge" for poliovirus genome-linked protein: isolation and characterization of viable and nonviable mutants. Proc. Natl. Acad. Sci. USA 85:519-523.

  • Wimmer, E., R. J. Kuhn, S. Pincus, C-F. Yang, H. Toyoda, M. Nicklin and N. Takeda. 1987. Molecular events leading to picornavirus genome replication, pp. 251-276. 1987. In Davies, J. W., R. Hull, K. F. Chater, T. H. N. Ellis, G. P. Lomonossoff, and H. W. Woolhouse (eds.), Virus Replication and Genome Interactions. 7th John Innes Symposium, Cambridge: Co. of Biologists Ltd.

  • Pincus, S. E., R. J. Kuhn, C-F. Yang, H. Toyoda, N. Takeda and E. Wimmer. 1987. The poliovirus genome: a unique RNA in structure, gene organization, and replication, pp. 175-220. In Inouye, M. and B. S. Dudock (eds.), Molecular Biology of RNA: New Perspectives. Academic Press.

  • Takeda, N., C-F. Yang, R. J. Kuhn and E. Wimmer. 1987. Uridylylation of the genome-linked protein of poliovirus is dependent upon an endogenous RNA template. Virus Research 8:193-204.

  • Paul, A. V., C-F. Yang, S. K. Jang, R. J. Kuhn, H. Tada, M. Nicklin, H. G. Krusslich, C. K. Lee and E. Wimmer. 1987. Molecular events leading to poliovirus genome replication, pp. 343-353. In Cold Spring Harbor Symp. Quant. Biology.

  • Kuhn, R. J. and E. Wimmer. 1987. The replication of picornaviruses, pp. 17-51. In Rowlands, D., J. M. A. Mayo, and B. W. J. Mahy (eds.), The Molecular Biology of the Positive Strand RNA Viruses. Academic Press.

  • Kuhn, R. J., E. Wimmer and B. L. Semler. 1987. Expression of the poliovirus genome from infectious cDNA is dependent upon arrangements of eukaryotic and prokaryotic sequences in recombinant plasmids. Virology 157:550-564.

  • Takeda, N., R. J. Kuhn, C-F. Yang, T. Takegami and E. Wimmer. 1986. Initiation of poliovirus plus strand RNA synthesis in a membrane complex of infected HeLa cells. J. Virol. 60:43-53.

  • Takegami, T., R. J. Kuhn, C. W. Anderson and E. Wimmer. 1983. Membrane-dependent uridylation of the genome-linked protein VPg of poliovirus. Proc. Natl. Acad. Sci. USA 80:7447-7451.